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Antimutagenic in vitro activity of plant polyphenols: Pycnogenol ® and Ginkgo biloba extract (EGb 761)
Author(s) -
Križková Lívia,
Chovanová Zuzana,
Ďuračková Zdenka,
Krajčovič Juraj
Publication year - 2008
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.2331
Subject(s) - ginkgo biloba , chemistry , acridine orange , ginkgoales , antioxidant , citrus paradisi , euglena gracilis , polyphenol , biochemistry , orange (colour) , pharmacognosy , food science , traditional medicine , pharmacology , in vitro , botany , biological activity , rutaceae , biology , medicine , apoptosis , chloroplast , gene
Abstract Ofloxacin (15 µg/mL) and acridine orange (5 µg/mL) induce mutagenicity by different mechanisms in the photosynthetic flagellate Euglena gracilis . The present study examined whether Pycnogenol ® (PYC; 5–100 µg/mL) or Ginkgo biloba extract (EGb 761; 5–100 µg/mL) could protect against the mutagenic effects of each of the mutagens and the potential mechanisms underlying such protection. The highest concentration of PYC and EGb 761 effectively reduced the mutagenic activity of both ofloxacin and acridine orange by more than 99% ( p < 0.001). Using luminol‐dependent photochemical methodology it was demonstrated that EGb 761 and PYC were effective antioxidants. In addition, as determined by spectrophotometry, PYC and EGb 761 bound acridine orange. Both PYC and EGb 761 have been shown to produce dual antimutagenic effects, as evidenced by both antioxidant and physicochemical properties. The findings suggest that EGb 761 and PYC would thus be suitable for future study, not only as antioxidants, but also as antimutagenic agents. Copyright © 2007 John Wiley & Sons, Ltd.