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Antiinflammatory activity of Lindera erythrocarpa fruits
Author(s) -
Wang ShengYang,
Lan XingYu,
Xiao JunHong,
Yang JengChung,
Kao YiTing,
Chang ShangTzen
Publication year - 2008
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.2289
Subject(s) - ethyl acetate , phytotherapy , in vivo , chemistry , in vitro , ethanol , croton , croton oil , nitric oxide , pharmacology , traditional medicine , lipopolysaccharide , pharmacognosy , cell culture , biochemistry , biological activity , medicine , biology , inflammation , immunology , alternative medicine , microbiology and biotechnology , organic chemistry , pathology , genetics
In this study, in vitro and in vivo antiinflammatory activities of fruits from Lindera erythrocarpa Makino were evaluated. The ethyl acetate soluble fraction derived from the ethanol extract of L. erythrocarpa fruits inhibited significantly nitric oxide (NO) production in lipopolysaccharide (LPS) induced NO in the murine macrophage cell line (RAW264.7) assay, the EC 50 being 16.35 µg/mL. Four compounds, including lucidone (1), cis / trans ‐methylludicone (2), methyl linderone (3) and linderone (4) were identified from the active fraction based on the bioactivity‐guided fractionation procedure. Of these lucidone possessed the strongest NO inhibitory activity with an EC 50 value of 4.22 µg/mL. Furthermore, results from the protein expression assay demonstrated that lucidone suppressed iNOS and COX‐2 protein expression in a dose‐dependent manner. Lucidone also provided antiinflammatory activity in the croton oil‐induced ear edema assay. When it was applied topically at a dosage of 0.5 and 1 mg per ear, the percent edema reduction in treated mice was 44% and 25%, respectively. The results obtained in this study indicated that lucidone has a good potential to be developed as an antiinflammation agent. Copyright © 2007 John Wiley & Sons, Ltd.