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Protective effect of Amomi semen extract on alloxan‐induced pancreatic β ‐cell damage
Author(s) -
Lee JiHyun,
Park JinWoo,
Kim JongSuk,
Park ByungHyun,
Rho HyeWon
Publication year - 2008
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.2271
Subject(s) - alloxan , pancreatic islets , beta cell , chemistry , semen , dna damage , endocrinology , hamster , medicine , reactive oxygen species , islet , biology , biochemistry , andrology , insulin , diabetes mellitus , dna
Abstract The protective effect of Amomi semen extract (ASE) on alloxan‐induced pancreatic β ‐cell damage was investigated in HIT T‐15 cells, a Syrian hamster pancreatic β ‐cell line. Alloxan caused pancreatic β ‐cell damage through the generation of reactive oxygen species (ROS), the elevation of cytosolic free Ca 2+ , DNA fragmentation and the decrease of cellular NAD + and ATP levels. All these effects of alloxan were significantly prevented by pretreatment with a water‐soluble extract of Amomi semen. Pretreatment with ASE in pancreatic islets isolated from mice, also significantly abolished the inhibition of glucose‐stimulated insulin secretion by alloxan. The results of this study provide evidence that ASE may have a protective activity on alloxan‐induced β ‐cell damage, and that the protective effect is primarily due to the inhibition of ROS generation by alloxan. Copyright © 2007 John Wiley & Sons, Ltd.