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Antiplatelet and antithrombotic activity of indole‐3‐carbinol in vitro and in vivo
Author(s) -
Park MinKyu,
Rhee YunHee,
Lee HyoJung,
Lee EunOk,
Kim KwanHyun,
Park MinJong,
Jeon ByungHun,
Shim BumSang,
Jung ChangHyun,
Choi SeungHoon,
Ahn KyooSeok,
Kim SungHoon
Publication year - 2008
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.2260
Subject(s) - antithrombotic , indole test , in vivo , pharmacology , thromboxane a2 , chemistry , thromboxane , platelet , in vitro , thromboxane b2 , prostaglandin , receptor , medicine , biochemistry , biology , microbiology and biotechnology
Indole‐3‐carbinol, a natural compound found in cruciferous vegetables, is known to have anticancer activity. In the present study, the antiplatelet and antithrombotic activities of indole‐3‐carbinol were investigated in vitro and in vivo . Indole‐3‐carbinol significantly inhibited collagen‐induced platelet aggregation in human platelet rich plasma (PRP) in a concentration‐dependent manner. Indole‐3‐carbinol significantly inhibited fibrinogen binding to the platelet surface glycoprotein IIb/IIIa (GP IIb/IIIa) receptor by flow cytometric analysis. In addition, the levels of thromboxane B 2 (TXB 2 ) and prostaglandin E 2 (PGE 2 ) in collagen stimulated PRP were significantly inhibited in a concentration‐dependent manner by indole‐3‐carbinol. Furthermore, indole‐3‐carbinol dose‐dependently suppressed the death of mice with pulmonary thrombosis induced by intravenous injection of collagen and epinephrine. These results suggest that indole‐3‐carbinol can be a potent antithrombotic agent with antiplatelet activity through the inhibition of GP IIb/IIIa receptor and thromboxane B 2 formation. Copyright © 2007 John Wiley & Sons, Ltd.