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Effect of grape seed extract on lipid peroxidation, antioxidant activity and peripheral blood lymphocytes in rats exposed to x‐radiation
Author(s) -
Enginar Hüseyin,
Cemek Mustafa,
Karaca Turan,
Unak Perihan
Publication year - 2007
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.2201
Subject(s) - lipid peroxidation , antioxidant , malondialdehyde , chemistry , glutathione , ascorbic acid , medicine , vitamin e , ceruloplasmin , grape seed extract , endocrinology , nitrite , vitamin , biochemistry , food science , nitrate , enzyme , pathology , alternative medicine , organic chemistry
The present studies were designed to evaluate supplemental grape seed extract (GSE) and vitamin E supplements on lipid peroxidation, on antioxidant systems and peripheral blood lymphocytes in rats exposed to x‐rays. Three groups of rats were investigated: a control group (CG) received intraperitoneal (i.p.) physiological serum 1 mL/day ( n = 10), i.p.; a vitamin E group (VG) received 50 mg/kg/day ( n = 10); an i.p. grape seed extract group received 50 mg/kg/day ( n = 10). Four weeks later, a 6 Gy radiation dose was given to the rats. Blood samples were taken 24 h later after irradiation and lymphocyte, malondialdehyde (MDA), reduced glutathione (GSH), nitrate, nitrite, reduced ascorbic acid, retinol, β ‐carotene and ceruloplasmin concentrations were analysed. The levels of GSH ( p < 0.05), retinol ( p < 0.001), β ‐carotene ( p < 0.05) and ceruloplasmin concentration ( p < 0.001) in the GSE group were found to be higher than in the control group but the level of MDA ( p < 0.001) and nitrite concentration ( p < 0.05) in rats supplemented with GSE were found to be lower than in the control group. The results indicate that GSE enhanced the antioxidant status and decreased the incidence of free radical‐induced lipid peroxidation in blood samples of rats exposed to x‐radiation. The antioxidant effect of GSE given to animals was more effective than vitamin E administered before whole‐body irradiation in rats. Copyright © 2007 John Wiley & Sons, Ltd.