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Antiinflammatory and antioxidant activities of Desmodium gangeticum fractions in carrageenan‐induced inflamed rats
Author(s) -
Govindarajan R.,
Vijayakumar M.,
Rao Ch. V.,
Shirwaikar A.,
Kumar Santhosh,
Rawat A. K. S.,
Pushpangadan P.
Publication year - 2007
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.2199
Subject(s) - carrageenan , antioxidant , pharmacognosy , traditional medicine , desmodium , chemistry , biology , medicine , biochemistry , in vitro , biological activity
Abstract Flavonoid and alkaloid fractions of Desmodium gangeticum were evaluated for antiinflammatory and antioxidant activities in carrageenan‐induced inflamed rats with the aim of studying the promising fraction for inhibitory action on ferrous sulphate induced lipid peroxidation, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX) and total reduced glutathione in liver and spleen homogenates of inflamed rats. The results showed that the flavonoid fraction of D. gangeticum possesses potent antioxidant activity compared with the alkaloid fraction and also with respect to the standard drug indomethacin, in terms of augmentation of the liver and spleen SOD, CAT and GPX activities, concomitant with a reduction in lipid peroxidation (TBARS). In addition, the quantification of caffeic and chlorogenic acid in the flavonoid fraction has also been carried out using HPLC, which can be utilized as a marker compound in the standardization. Copyright © 2007 John Wiley & Sons, Ltd.