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Phlomis umbrosa root inhibits mast cell‐dependent allergic reactions and inflammatory cytokine secretion
Author(s) -
Shin TaeYong,
Kim SangHyun,
Kim DaeKeun,
Leem KangHyun,
Park JeongSuk
Publication year - 2008
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.2164
Subject(s) - histamine , mast cell , cytokine , immunoglobulin e , secretion , pharmacology , interleukin 4 , phorbol , chemistry , immunology , endocrinology , medicine , biochemistry , antibody , protein kinase c , signal transduction
The effect of an aqueous extract of Phlomis umbrosa Turcz. (Labiatae) root (PUAE) on mast cell‐dependent allergic reactions and inflammatory cytokine secretion were investigated. PUAE (0.01–1 g/kg) inhibited compound 48/80‐induced systemic allergic reaction. When PUAE was employed in a systemic allergic reaction test, the plasma histamine levels were reduced in a dose‐dependent manner. PUAE (0.1 and 1 g/kg) also significantly inhibited the local allergic reaction activated by anti‐dinitrophenyl (DNP) IgE. PUAE (0.001–1 mg/mL) dose‐dependently inhibited the histamine release from rat peritoneal mast cells activated by compound 48/80 or anti‐DNP IgE. PUAE (0.01–1 mg/mL) inhibited the secretion of interleukin (IL)‐1 β in phorbol 12‐myristate 13‐acetate plus calcium ionophore A23187‐stimulated human mast cell line (HMC‐1) cells. PUAE (1 mg/mL) inhibited the gene expression and production of the main inflammatory cytokine, TNF‐ α , in HMC‐1 cells. These results provide evidence that PUAE may be beneficial in the treatment of allergic diseases. Copyright © 2007 John Wiley & Sons, Ltd.