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Pycnogenol ® reduces talc‐induced neoplastic transformation in human ovarian cell cultures
Author(s) -
Buz'Zard Amber R.,
Lau Benjamin H. S.
Publication year - 2007
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.2117
Subject(s) - talc , malignant transformation , neoplastic transformation , carcinogenesis , ovarian cancer , carcinogen , ovary , cancer research , reactive oxygen species , cell growth , biology , medicine , pharmacology , chemistry , traditional medicine , endocrinology , cancer , microbiology and biotechnology , biochemistry , paleontology
Talc and poor diet have been suggested to increase the risk of developing ovarian cancer; which can be reduced by a diet rich in fruit and vegetables. Talc is ubiquitous despite concern about its safety, role as a possible carcinogen and known ability to cause irritation and inflammation. It was recently shown that Pycnogenol ® (Pyc; a proprietary mixture of water‐soluble bioflavonoids extracted from French maritime pine bark) was selectively toxic to established malignant ovarian germ cells. This study investigated talc‐induced carcinogenesis and Pyc‐induced chemoprevention. Normal human epithelial and granulosa ovarian cell lines and polymorphonuclear neutrophils (PMN) were treated with talc, or pretreated with Pyc then talc. Cell viability, reactive oxygen species (ROS) generation and neoplastic transformation by soft agar assay were measured. Talc increased proliferation, induced neoplastic transformation and increased ROS generation time‐dependently in the ovarian cells and dose‐dependently in the PMN. Pretreatment with Pyc inhibited the talc‐induced increase in proliferation, decreased the number of transformed colonies and decreased the ROS generation in the ovarian cells. The data suggest that talc may contribute to ovarian neoplastic transformation and Pyc reduced the talc‐induced transformation. Taken together, Pyc may prove to be a potent chemopreventative agent against ovarian carcinogenesis. Copyright © 2007 John Wiley & Sons, Ltd.