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A Fraction of Acorus calamus L. extract devoid of β ‐asarone Enhances adipocyte differentiation in 3T3‐L1 cells
Author(s) -
Wu Haoshu,
Li Yingyin,
Weng Linjia,
Zhou Changxin,
He Qiaojun,
Lou Yijia
Publication year - 2007
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.2112
Subject(s) - glut4 , rosiglitazone , acorus calamus , adipocyte , 3t3 l1 , glucose transporter , adipogenesis , chemistry , ethyl acetate , biochemistry , insulin , pharmacology , biology , in vitro , endocrinology , botany , adipose tissue , rhizome
The effects of fractions partitioned from the ethanol extract of Acorus calamus L. (AC) on adipocyte differentiation were investigated using cultured mouse 3T3‐L1 preadipocytes. The degree of differentiation was evaluated by measuring the cellular triglycerides and protein expression of the glucose transporter GLUT4 in 3T3‐L1 cells. The ethyl acetate fraction of the AC extract (ACE) was found to enhance adipocyte differentiation as did rosiglitazone. The results of further fractionation of ACE indicated that the active fraction does not consist of β ‐asarone, which is a toxic component of this plant. This finding suggests that ACE has potential insulin‐sensitizing activity like rosiglitazone, and may improve type 2 diabetes. Copyright © 2007 John Wiley & Sons, Ltd.