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Carbenoxolone and triterpenoids inhibited mucin secretion from airway epithelial cells
Author(s) -
Heo Ho Jin,
Kim Cheolsu,
Lee Hyun Jae,
Kim Young Sik,
Kang Sam Sik,
Seo Un Kyo,
Kim Yun Hee,
Park Yang Chun,
Seok Jeong Ho,
Lee Choong Jae
Publication year - 2007
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.2102
Subject(s) - carbenoxolone , mucin , oleanolic acid , secretion , ursolic acid , mucus , cytotoxicity , lactate dehydrogenase , biochemistry , respiratory epithelium , chemistry , biology , pharmacology , epithelium , medicine , in vitro , intracellular , pathology , gap junction , ecology , alternative medicine , genetics , chromatography , enzyme
This study investigated whether carbenoxolone, oleanolic acid and ursolic acid affect ATP‐induced mucin secretion from cultured airway epithelial cells. Confluent primary hamster tracheal surface epithelial (HTSE) cells were metabolically radiolabeled using 3 H‐glucosamine for 24 h and chased for 30 min in the presence of varying concentrations of each agent to assess the effects on 3 H‐mucin secretion. The possible cytotoxicity of each agent was investigated with a lactate dehydrogenase assay. The results were as follows: (1) carbenoxolone, oleanolic acid and ursolic acid significantly inhibited the secretion of airway mucin induced by ATP; (2) none of the compounds showed significant cytotoxicity at any concentration. This result suggests that carbenoxolone, oleanolic acid and ursolic acid can regulate ‘mucin secretion induced by ATP’ – a phenomenon simulating mucus overproduction from inflamed airway epithelial cells – by directly acting on airway mucin‐secreting cells. Copyright © 2007 John Wiley & Sons, Ltd.