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Presence of cholinergic and calcium antagonist constituents in Saussurea lappa explains its use in constipation and spasm
Author(s) -
Gilani Anwar H.,
Shah Abdul J.,
Yaeesh S.
Publication year - 2007
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.2098
Subject(s) - cholinergic , antagonist , contraction (grammar) , acetylcholine , ileum , chemistry , calcium , jejunum , inhibitory postsynaptic potential , traditional medicine , pharmacology , calcium channel , constipation , muscarinic acetylcholine receptor , medicine , biochemistry , receptor
This study was carried out to provide a scientific basis for the traditional use of Saussurea lappa , in constipation and spasms. Isolated tissue preparations were used to see if the aqueous‐methanol crude extract of the S. lappa root (Sl.Cr) contains gut stimulatory and inhibitory constituents. In isolated guinea‐pig ileum, a quiescent preparation, Sl.Cr caused a concentration‐dependent (0.3–5.0 mg/mL) spasmogenic effect, with the maximum effect reaching 91% of the acetylcholine maximum. A further increase in concentration caused a declining effect, indicating the presence of spasmolytic constituent(s). The spasmolytic effect was more marked in the spontaneously contracting rabbit jejunum and in the atropinized preparations. The spasmolytic effect was mediated through calcium channel blocking (CCB) activity, as evident by its inhibitory effect against high K + (80 m m )‐induced contraction and displacement of the Ca ++ concentration‐response curves to the right. These data indicate that the crude extract of Sl.Cr contains gut stimulatory constituent(s) of cholinergic‐type providing a scientific basis for its use in constipation. The presence of spasmolytic constituents of CCB‐type more evident in the spontaneous contracting gut preparation may explain its use in spasms. Copyright © 2007 John Wiley & Sons, Ltd.