Premium
Isolation of coumarins and ferulate from the roots of Angelica purpuraefolia and the antitumor activity of khellactone
Author(s) -
Lee HyeongKyu,
Oh SeiRyang,
Kwon OkKyoung,
Ahn KyoungSeop,
Lee Joongku,
Kim JinCheol,
Min ByungSun,
Joung Hyouk
Publication year - 2007
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.2082
Subject(s) - coumarin , stereochemistry , chemistry , human lung , plant roots , pharmacology , ratón , lung , biology , medicine , immunology , botany , organic chemistry
A new coumarin, hydroxylomatin (1), was isolated from the CHCl 3 ‐soluble fraction of the roots of Angelica purpuraefolia , along with one ferulate (2) and three other known coumarins (3–5) including khellactone (3). The structure of hydroxylomatin (1) was determined to be 3′ β ,5′‐dihydroxy‐3′,4′‐dihydroseselin (1) by spectroscopic means including 2D‐NMR. The modified Mosher's method was used to determine the chiral center at C‐1 of compound 2. Khellactone (3) is a major compound of the roots of A. purpuraefolia . This study also examined the antitumor activity of khellactone (3) using a LLC mouse lung carcinoma in the BDF‐1 mice and a NCI‐H460 human lung carcinoma in a human tumor xenograft model in nude mice. This compound (3) inhibited LLC tumor growth with a T / C (mean value of treated group/mean value of control group) value of 12.9% at a dose of 5 mg/kg and 33.2% at a dose of 10 mg/kg, respectively, in a dose‐dependent manner. In addition, it suppressed the growth of NCI‐H460 tumor cells, accounting for 81.4% at a dose of 10 mg/kg in nude mice. Copyright © 2007 John Wiley & Sons, Ltd.