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In Vitro effect of D‐004, a lipid extract of the ground fruits of the cuban royal palm ( Roystonea regia ), on rat microsomal lipid peroxidation
Author(s) -
Menéndez Roberto,
Más Rosa,
Pérez Yohani,
González R. M.
Publication year - 2007
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.2012
Subject(s) - lipid peroxidation , antioxidant , microsome , chemistry , biochemistry , superoxide , in vitro , in vivo , abts , hydroxyl radical , oxidative stress , dpph , biology , enzyme , microbiology and biotechnology
D‐004 is a lipid extract of the fruits of the Cuban royal palm ( Roystonea regia ) containing a mixture of saturated fatty acids. D‐004 inhibits prostate hyperplasia (PH) induced with testosterone, in rodents. Since fatty acids inhibit lipid peroxidation (LP), this study investigated whether D‐004 prevents in vitro LP. D‐004 (0.9–1000 µg/mL) markedly and dose‐dependently inhibited in vitro iron‐induced LP in native brain and liver microsomes. D‐004 showed hydroxyl radical scavenging activity, which could explain partially its antioxidant effect on microsomal iron‐induced LP, but it was unable to scavenge superoxide and ABTS radicals, indicating a limited radical scavenging activity. Also, D‐004 inhibited CCl4‐mediated LP in active liver microsomes through a decreased generation of radical species rather than a radical trapping action on CCl 4 ‐derived radical species. D‐004 also inhibited lipooxygenase (LOX) and cyclooxygenase (COX) activities, and the generation of protein‐associated carbonyl groups after LP. Since increased oxidative stress has been linked to PH, the antioxidant effect of D‐004 shown here could contribute to explaining its beneficial effects on T‐induced PH in rodents. Nevertheless, this study shows only in vitro results. Further studies should investigate whether D‐004 also exhibits antioxidant effects in vivo . Copyright © 2006 John Wiley & Sons, Ltd.