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CKBM stimulates MAPKs but inhibits LPS‐induced IFN‐ γ in lymphocytes
Author(s) -
Chan Anthony S.L.,
Yip Eric C.H.,
Yung Lisa Y.,
Pang Haihong,
Luk Sharon C.W.,
Pang Shiu F.,
Wong Yung H.
Publication year - 2006
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.1943
Subject(s) - p38 mitogen activated protein kinases , mapk/erk pathway , cytokine , intracellular , peripheral blood mononuclear cell , kinase , pharmacology , protein kinase a , mitogen activated protein kinase , microbiology and biotechnology , biology , medicine , chemistry , immunology , biochemistry , in vitro
CKBM is an herbal formula composed of five Chinese medicinal herbs ( Panax ginseng , Schisandra chinensis , Fructus crataegi , Ziziphus jujuba and Glycine max ) supplemented with processed Saccharomyces cerevisiae . It has been demonstrated that CKBM is capable of triggering the release of IL‐6 and TNF α from human peripheral blood mononuclear cells. In this report, T‐lymphocytic Sup‐T1 cells and B‐lymphocytic Ramos cells were utilized as cellular models to investigate how CKBM regulates intracellular signaling as well as the production of cytokines. CKBM stimulated the three major subgroups of mitogen‐activated protein kinase (i.e. ERK, JNK and p38) in Sup‐T1 cells, but only triggered the activation of ERK and p38 in Ramos cells. The induction of mitogen‐activated protein kinases (MAPK) activations varied with the duration of treatment, as well as with the dosage of CKBM. In terms of cytokine production, treatment of CKBM alone did not trigger the release of IL‐1 β and IFN γ , but it suppressed the LPS‐induced IFN γ production from both Sup‐T1 cells and Ramos cells. In view of the therapeutic effects of traditional Chinese medicines in inflammatory and autoimmune disorders, the results suggest that CKBM may exhibit its immuno‐modulatory effects by regulating intracellular signaling as well as cytokine production in different lymphocytic cell types. Copyright © 2006 John Wiley & Sons, Ltd.