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Antibacterial activity of components from Lomatium californicum
Author(s) -
Chou ShenChieh,
Everngam Molly C.,
Sturtz George,
Beck John J.
Publication year - 2006
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.1828
Subject(s) - high performance liquid chromatography , chromatography , bacillus subtilis , column chromatography , dept , ferulic acid , antibacterial activity , chemistry , ethyl acetate , antibacterial agent , bacteria , stereochemistry , biology , biochemistry , antibiotics , genetics
Abstract The isolation, characterization and bioactivity testing of compounds from Lomatium californicum (Nutt.) are described. Ethyl acetate and hexane extracts of the roots of L. californicum were subjected to vacuum liquid chromatography (VLC), flash column chromatography (FCC) and separation by normal‐ and reverse‐phase high‐performance liquid chromatography (HPLC). Six compounds were isolated successfully and characterized by 1D and 2D nuclear magnetic resonance (NMR) experimentation. The bioactivity of the known compounds (+)‐falcarindiol, coniferyl ferulate, ferulic acid and ( Z )‐ligustilide were conrmed against the Gram‐positive bacteria Bacillus subtilis and Staphylococcus aureus . The known compounds senkyunolide I and trans‐ neocnidilide were also isolated but in too small a quantity for similar testing. Copyright © 2006 John Wiley & Sons, Ltd.