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Protective effect of selected flavonoids on in vitro daunorubicin‐induced cardiotoxicity
Author(s) -
Mojžišová G.,
Mirossay L.,
Kučerová D.,
Kyselovič J.,
Miroššay A.,
Mojžiš J.
Publication year - 2006
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.1811
Subject(s) - trolox , daunorubicin , quercetin , naringenin , pharmacology , chemistry , antioxidant , lactate dehydrogenase , flavonoid , toxicity , cardiotoxicity , biochemistry , medicine , enzyme , chemotherapy , organic chemistry , dpph
Flavonoids are an ubiquitous group of polyphenolic substances with varied chemical structures present in foods of plant origin and act as free radical scavenging and chelating agents with a variety of biological activities. Using a model of spontaneously beating, cultured adult rat cardiomyocytes, this study examined the cardioprotective role of quercetin, naringenin, pycnogenol and a model antioxidant, trolox, against daunorubicin‐induced toxicity. Cardiomyocyte protection was assessed by MTT test and extracellular lactate dehydrogenase detection. Protection of cardiomyocytes was concentration/dose dependent for quercetin > naringenin > pycnogenol > trolox. Quercetin (10 −4 –10 −6 mol/L) after 24 h of co‐incubation with daunorubicin significantly increased the cardiomyocyte survival in all tested concentrations ( p < 0.001). The cytoprotective effect of naringenin (10 −4 –10 −6 mol/L) was similar to those of quercetin ( p < 0.001 and p < 0.01, respectively). Pycnogenol was the least effective of the flavonoids studied. On the other hand, all tested flavonoids had significantly better protective effects than trolox. The leakage of lactate dehydrogenase induced by daunorubicin was also prevented by the studied compounds and was in accordance with their cytoprotective activity. Copyright © 2006 John Wiley & Sons, Ltd.