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Prevention of oxidative injury by flavonoids from stems and leaves of Scutellaria Baicalensis georgi in PC12 cells
Author(s) -
Shang YaZhen,
Qin BoWen,
Cheng JianJun,
Miao Hong
Publication year - 2006
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.1802
Subject(s) - scutellaria baicalensis , neuroprotection , superoxide dismutase , lactate dehydrogenase , malondialdehyde , pharmacology , reactive oxygen species , oxidative stress , chemistry , biochemistry , medicine , enzyme , pathology , alternative medicine , traditional chinese medicine
Reactive oxygen species (ROS) are important mediators in a number of neurodegenerative diseases, such as Alzheimer's disease (AD) and Parkinson's disease (PD). The neuroprotective effects of flavonoids from the stems and leaves of Scutellaria baicalensis Georgi (SSF) against hydrogen peroxide (H 2 O 2 )‐induced rat pheochromocytoma line PC12 injury were evaluated by cell lesion, free radicals and ATPase disorders. Following a 30 min exposure of the cells to H 2 O 2 (100 µ m ), a marked decrease in cell survival and activity of superoxide dismutase (SOD) and Na + ‐K + ‐ATPase as well as an increase of malondialdehyde (MDA) production and lactate dehydrogenase (LDH) release were observed. Pretreatment of the cells with SSF (18–76 µg/mL) prior to H 2 O 2 exposure notably elevated the cell survival and activity of SOD and Na + ‐K + ‐ATPase, and lowered the MDA level and LDH release. Neuroprotection by SSF was also observed in animal models. The present results indicated that SSF exerts neuroprotective effects against H 2 O 2 toxicity, which might be of importance and might contribute to its clinical efficacy for the treatment of neurodegenerative disease. Copyright © 2006 John Wiley & Sons, Ltd.