Premium
Antioxidant activity of Plantago bellardii All.
Author(s) -
Gálvez Marina,
MartínCordero Carmen,
Houghton Peter J.,
Ayuso María Jesús
Publication year - 2005
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.1797
Subject(s) - rutin , verbascoside , antioxidant , dpph , chemistry , lipid peroxidation , high performance liquid chromatography , chromatography , traditional medicine , colorimetry , iridoid , biochemistry , organic chemistry , medicine , glycoside
The aim of this study was to evaluate the in vitro antioxidant activity of the methanol extract of Plantago bellardii All. aerial parts. This was assessed by two different tests, scavenging of 1,1‐diphenyl‐2‐picrylhydrazil (DPPH) radical, and inhibition of lipid peroxidation on liposomes prepared from bovine brain extract. In both tests the extract showed a potent antioxidant effect. The characterization of the major compounds in the extract as rutin, geniposide and verbascoside was performed by isolation and HPLC comparison with authentic samples. They were quantified by HPLC for the flavonoids and colorimetry for iridoids. The compounds that contribute most to the antioxidant activity were shown to be verbascoside and rutin. Copyright © 2005 John Wiley & Sons, Ltd.