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Aged garlic extract induces proliferation and ameliorates gentamicin‐induced toxicity in LLC‐PK1 cells
Author(s) -
VelascoVelázquez Marco A.,
Maldonado Perla D.,
Barrera Diana,
Torres Víctor,
ZentellaDehesa Alejandro,
PedrazaChaverrí José
Publication year - 2006
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.1780
Subject(s) - genotoxicity , nephrotoxicity , toxicity , cell growth , viability assay , gentamicin , pharmacology , biology , chemistry , toxicology , in vitro , biochemistry , antibiotics , organic chemistry
Gentamicin (GM)‐induced nephrotoxicity limits the use of this antibiotic. It has been shown that aged garlic extract (AGE) and S‐allylcysteine (SAC), the most abundant organosulfur compound in AGE, ameliorate GM‐induced nephrotoxicity in rats. The present communication evaluated the effect of AGE and SAC on proliferation and on GM‐induced toxicity and genotoxicity of porcine kidney epithelial cell line (LLC‐PK1 cells). The cells were preincubated with different concentrations of AGE or SAC for 12 h before incubation with 8 m m GM for an additional 72 h. At the end of this time, cell viability, genotoxicity and proliferation were evaluated. AGE stimulated cell proliferation and protected LLC‐PK1 cells from GM‐mediated toxicity and genotoxicity. SAC partially prevented only GM‐induced genotoxicity. These results suggest that the stimulation of cell proliferation could possibly be one of the mechanisms involved in the in vitro protective effect of AGE in GM‐induced toxicity of LLC‐PK1 cells. Copyright © 2006 John Wiley & Sons, Ltd.