Premium
Assessment of a bioactive compound for its potential antiinflammatory property by tight junction permeability
Author(s) -
Bai Young Hoon,
Pak Sok Cheon,
Lee Seung Hoo,
Bae Chun Sik,
Prosser Colin,
Stelwagen Kerst,
Lee Jin Hee,
Park Soon Duck
Publication year - 2005
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.1772
Subject(s) - permeability (electromagnetism) , tight junction , chemistry , property (philosophy) , traditional medicine , pharmacology , stereochemistry , medicine , biochemistry , philosophy , membrane , epistemology
Lactobacillus probiotic strains are proving to be abundant sources of bioactive components, including antiinflammatory components. Lifree was made of fruits fermented by Lactobacillus paracasei , Lactobacillus reuterrii and Saccharomyces cerevisiae . This study was designed to test these compounds in cell assays measuring epithelial barrier function and proliferation in the first instance. Cell proliferation was measured in mouse fibroblasts cells (3T3NIH) and rat intestinal epithelial cells (IEC‐6), and tight junction activity in the kidney epithelial cell line (MDCK). Tight junction permeability was assessed by measuring transepithelial electrical resistance (TER) across confluent monolayers, following the addition of Lifree with or without a challenge with EGTA. Lifree promoted tight junction formation and recovery following loss of TER from challenge with EGTA. On the other hand, Lifree did not stimulate cell growth in either 3T3NIH and IEC‐6 cells. Lifree stimulates tight junction maintenance and formation, suggesting it may have potential antiinflammatory properties. Copyright © 2005 John Wiley & Sons, Ltd.