Premium
Bio‐guided isolation of cholinesterase inhibitors from the bulbs of
Author(s) -
Kissling Jonathan,
Ioset JeanRobert,
Marston Andrew,
Hostettmann Kurt
Publication year - 2005
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.1770
Subject(s) - cholinesterase , isolation (microbiology) , traditional medicine , pharmacognosy , medicine , pharmacology , biology , biological activity , bioinformatics , biochemistry , in vitro
In a search for potential acetylcholinesterase (AChE) inhibitors, the ethanol extract of the bulbs of Crinum × powellii (Amaryllidaceae) was found to demonstrate a marked inhibition of this enzyme. Using a bio‐guided isolation strategy, linoleic acid ethyl ester has been identified as the compound responsible for this inhibition. Three other molecules – the alkaloid hippadine, the glycosylated benzyl alcohol derivative calleryanin and 4′‐hydroxy‐7‐methoxyflavan – were also isolated and characterized for the first time from Crinum x powellii. The structures of these compounds were elucidated by spectrometric methods including EI, D/CI mass spectrometry, 1 H, 13 C and 2D NMR experiments. Linoleic acid was also found to inhibit AChE. Copyright © 2005 John Wiley & Sons, Ltd.