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Evaluation of flavonoids from Bauhinia megalandra leaves as inhibitors of glucose‐6‐phosphatase system
Author(s) -
Estrada Omar,
Hasegawa Masahisa,
GonzalezMujíca Freddy,
Motta Norma,
Perdomo Elsa,
Solorzano Alejandra,
Méndez Jeannette,
Méndez Bernardo,
Zea E Gabriela
Publication year - 2005
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.1703
Subject(s) - quercetin , kaempferol , microsome , biochemistry , pharmacognosy , ic50 , flavonoid , chemistry , traditional medicine , biology , biological activity , enzyme , antioxidant , in vitro , medicine
From the methanol extract of Bauhinia megalandra fresh leaves, eight flavonoids were isolated and evaluated by rat liver microsomal glucose‐6‐phosphatase (G‐6‐Pase) bioassay, which might be a useful methodology for screening antihyperglycaemic substances. All the flavonoids assayed showed an inhibitory effect on the intact microsomal G‐6‐Pase: quercetin and kaempferol exhibited the lowest effect; astilbin, quercetin 3‐ O ‐ α ‐rhamnoside, kaempferol 3‐ O ‐ α ‐rhamnoside and quercetin 3‐ O ‐ α ‐arabinoside an intermediate effect. The highest inhibitory activity was shown by quercetin 3‐ O ‐ α ‐(2″‐galloyl)rhamnoside and kaempferol 3‐ O ‐ α ‐(2″galloyl)rhamnoside. None of the flavonoids mentioned above showed an inhibitory effect on the disrupted microsomal G‐6‐Pase. Quercetin 3‐ O ‐ α ‐(2″‐galloyl)rhamnoside and kaempferol 3‐ O ‐ α ‐(2″‐galloyl)rhamnoside exhibited the lowest IC 50 of all the flavonoids assayed. Also, the phlorizin IC 50 is reported. Copyright © 2005 John Wiley & Sons, Ltd.