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Cardamom extract as inhibitor of human platelet aggregation
Author(s) -
Suneetha W. Jessie,
Krishnakantha T. P.
Publication year - 2005
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.1681
Subject(s) - platelet , lipid peroxidation , malondialdehyde , chemistry , ascorbic acid , epinephrine , ionophore , pharmacology , biochemistry , platelet rich plasma , calcium , endocrinology , medicine , antioxidant , membrane , food science , organic chemistry
The inhibitory activity of cardamom extract was studied on human platelets. Platelet aggregation and lipid peroxidation were evaluated with platelet rich plasma (PRP) and platelet membranes, respectively, obtained from blood of healthy volunteers. Human platelets were subjected to stimulation with a variety of agonists including ADP (2.5 m m ), epinephrine (2.5 m m ), collagen (10 m m ), calcium ionophore A 23187 (6 µ m ) and ristocetin (1.25 µg/mL). The IC 50 were 0.49, 0.21, 0.55 and 0.59 mg with ADP, epinephrine, collagen and calcium ionophore A 23187, respectively, and no inhibition with ristocetin. The inhibitory effect was dose dependent with concentrations varying between 0.14 and 0.70 mg and time dependent at IC 50 . Lipid peroxidation induced by iron – ascorbic acid system in platelet membranes was analysed with malondialdehyde (MDA) as an index. An increase in concentration of cardamom has decreased the MDA formation significantly. Hence, it may be said that aqueous extract of cardamom may have component(s), which protect platelets from aggregation and lipid peroxidation. Copyright © 2005 John Wiley & Sons, Ltd.