Premium
In vitro antimycotic activity of some plant extracts towards yeast and yeast‐like strains
Author(s) -
Turchetti B.,
Pinelli P.,
Buzzini P.,
Romani A.,
Heimler D.,
Franconi F.,
Martini A.
Publication year - 2005
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.1622
Subject(s) - yeast , camellia sinensis , cryptococcus neoformans , biology , candida glabrata , traditional medicine , polyphenol , epigallocatechin gallate , botany , pharmacognosy , food science , microbiology and biotechnology , chemistry , biochemistry , in vitro , antifungal , biological activity , antioxidant , medicine
As part of screening aimed at the selection of novel antimycotic compounds of vegetable origin, leaf extracts of Camellia sinensis L., Cupressus sempervirens L. and Pistacia lentiscus L. and the seed extract of Glycine soja Sieb. et Zucc. were tested against yeast and yeast‐like species implicated in human mycoses. Of the extracts only those of C. sinensis (obtained from a commercial preparation of green tea) exhibited broad activity towards Candida glabrata , Clavispora lusitatiae , Cryptococcus laurentii , Filobasidiella neoformans , Issatchenkia orientalis , Saccharomyces cerevisiae and Prototheca wickerhamii strains. MICs ranging from 300 to 4800 µg extract[sol ]mL (corresponding to 130–2010 µg[sol ]mL total polyphenols) were observed. Concentrations of the C. sinensis extract over 25 000 µg[sol ]mL caused a rapid decrease of viable cells of Fil. neoformans and its activity was dose‐dependent. Tests carried out using the pure polyphenols present in C. sinensis extract composition, showed that only epicatechin‐3‐O‐gallate (ECG) and epigallocatechin‐3‐O‐gallate (EGCG) possess antimycotic activity. Copyright © 2005 John Wiley & Sons, Ltd.