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Effects of green tea catechin‐induced lipolysis on cytosol glycerol content in differentiated 3T3‐L1 cells
Author(s) -
Mochizuki Miyako,
Hasegawa Noboru
Publication year - 2004
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.1580
Subject(s) - lipolysis , glycerol , catechin , cytosol , incubation , chemistry , triglyceride , epigallocatechin gallate , biochemistry , 3t3 l1 , food science , polyphenol , adipocyte , adipose tissue , antioxidant , enzyme , cholesterol
The inuence of catechins in green tea on lipolysis in fully differentiated 3T3‐L1 cells was studied and glycerol release into the buffer, cytosol and residual triglyceride were measured. The addition of (‐)‐epigallocatechin‐3‐gallate (EGCG) stimulated glycerol release into the cytosol signicantly after incubation for 4 h, but had no effect on that into the buffer. However, (+)‐catechins did not produce a signicant increase in lipolysis. These data suggested that EGCG has strong lipolytic activity. Copyright © 2004 John Wiley & Sons, Ltd.