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A promising peptide antibiotic from Terfezia claveryi aqueous extract against Staphylococcus aureus in vitro
Author(s) -
Janakat S.,
AlFakhiri S.,
Sallal A.K.
Publication year - 2004
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.1563
Subject(s) - antimicrobial , sephadex , staphylococcus aureus , chemistry , aqueous solution , chromatography , antibiotics , ammonium , antibacterial activity , peptide , in vitro , traditional medicine , biochemistry , bacteria , biology , organic chemistry , medicine , genetics , enzyme
The antimicrobial activity of aqueous and methanolic extracts, as well as partially puried proteins extracted from Terfezia claveryi aqueous extract were investigated against Staphylococcus aureus in vitro . A 5% aqueous extract inhibited the growth of S. aureus by 66.4%, while a methanolic extract was ineffective. Partial protein purication of the aqueous extract using ammonium sulphate precipitation revealed that antimicrobial activity was within the third fraction. This fraction was then subjected to gel ltration using Sephadex G‐100. Two peaks were obtained. Peak one possessed higher antimicrobial activity. This peak was then subjected to ion exchange chromatography using DEAE Sephadex. Only peak 4 from the six peaks obtained showed a slight antimicrobial activity. Antimicrobial activities of the aqueous extract and the fractions that showed antimicrobial activity were compared with reference antibiotics. Copyright © 2004 John Wiley & Sons, Ltd.