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Suppressive effect of inducible nitric oxide synthase (iNOS) expression by the methanol extract of Actinodaphne lancifolia
Author(s) -
Kim Youngleem,
Min HyeYoung,
Park HyenJoo,
Lee EunJin,
Park EunJung,
Hwang HyeJin,
Jin Changbae,
Lee Yong Sup,
Lee Sang Kook
Publication year - 2004
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.1558
Subject(s) - nitric oxide synthase , nitric oxide , lipopolysaccharide , inflammation , chemistry , pharmacology , biochemistry , ic50 , enzyme , biology , in vitro , immunology , organic chemistry
Nitric oxide (NO) produced by inducible nitric oxide synthase (iNOS) has played a crucial role in various pathophysiological processes including inammation and carcinogenesis. Therefore, the inhibitors of NO synthesis or iNOS gene expression have been considered as potential anti‐inammatory and cancer chemopreventive agents. In our continuous search for iNOS inhibitors from natural products we have evaluated indigenous Korean plant extracts using an assay for inhibition of nitric oxide formation on lipopolysaccharide (LPS)‐activated mouse macrophage RAW 264.7 cells. As a result, the methanolic stem extract of Actinodaphne lancifolia showed an inhibitory activity of NO production in a dose‐dependent manner (IC 50 = 2.5 µg/ml). Additional study demonstrated that the extract of Actinodaphne lancifolia signicantly suppressed the iNOS protein and gene expression in a dose‐dependent manner. These results suggest that Actinodaphne lancifolia could be a potential candidate for developing an iNOS inhibitor from natural products. Further elucidation of active principles for development of new cancer chemopreventive and/or anti‐inammatory agents could be warranted. Copyright © 2004 John Wiley & Sons, Ltd.