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Antioxidant activity of supercritical extract of Melissa officinalis subsp. officinalis and Melissa officinalis subsp. inodora
Author(s) -
Marongiu Bruno,
Porcedda Silvia,
Piras Alessandra,
Rosa Antonella,
Deiana Monica,
Dessì Maria Assunta
Publication year - 2004
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.1549
Subject(s) - melissa officinalis , autoxidation , antioxidant , chemistry , linoleic acid , supercritical carbon dioxide , supercritical fluid , extraction (chemistry) , chromatography , food science , organic chemistry , botany , biology , fatty acid
Abstract The antioxidant activity of Melissa ofcinalis subsp. ofcinalis and of Melissa ofcinalis subsp. inodora extracts, obtained by using carbon dioxide under supercritical conditions was investigated. The samples were prepared in two steps. A preliminary extraction at 90 bar and 50 °C eliminated the essential oil, then a further extraction at 300 bar and 50 °C obtained the high molecular mass extract. These samples were tested for autoxidation and the iron or EDTA‐mediated oxidation of linoleic acid at 37 °C in the absence of solvent, in in vitro systems. During linoleic acid autoxidation and its EDTA‐mediated oxidation both M. ofcinalis and M. inodora extracts showed an antioxidant activity, and no signicant differences in their efcacy were observed. None showed any prooxidant activity. Copyright © 2004 John Wiley & Sons, Ltd.