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Antioxidant effect of EGb 761 on hydrogen peroxide‐induced lipoperoxidation of G‐6‐PD deficient erythrocytes
Author(s) -
Sarikçioğlu Süreyya Bïlmen,
Öner Gülsen,
Tercan Evren
Publication year - 2004
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.1544
Subject(s) - tbars , antioxidant , chemistry , thiobarbituric acid , oxidative stress , vitamin e , vitamin c , hydrogen peroxide , lipid peroxidation , biochemistry , pharmacology , endocrinology , medicine
In order to reduce the haemolytic susceptibility of glucose‐6‐phosphate dehydrogenase (G‐6‐PD) decient erythrocytes, Ginkgo biloba extract (EGb 761) and vitamin E (vit E) were used as antioxidant agents and their effects compared. The erythrocyte suspensions from control and G‐6‐PD decient patients were subjected to hydrogen peroxide (H 2 O 2 ) incubation for 1 h. The produced thiobarbituric acid reactive substance (TBARS) levels measured as nmol/g Hb were compared with those of the erythrocytes administered 250 µg/mL EGb 761 or vit E previously or concomitantly with H 2 O 2 . Preincubation with EGb 761 reduced the TBARS levels from 317.14 ± 25.27 to 160.09 ± 21.97 nmol/g Hb in controls and from 348.24 ± 7.79 to 205.60 ± 14.22 nmol/g Hb in decient erythrocytes. Concomitant application of EGb 761 with H 2 O 2 resulted in similar but less reduction. The antioxidative effects of vitamin E were comparable to those of EGb 761. Contrary to the results obtained from oxidant conditions, the antioxidant characteristics of EGb 761 and vitamin E were not observed when they were applied directly to the erythrocytes without oxidative stress. The ndings demonstrate that irrespective of administration time, EGb 761 signicantly reduced TBARS levels in the erythrocytes of control and G‐6‐PD decient patients subjected to oxidative stress. Copyright © 2004 John Wiley & Sons, Ltd.