Anti‐HIV‐1 activities in extracts from some medicinal plants as assessed in an  in vitro  biochemical HIV‐1 reverse transcriptase assay | Zendy

Kanyara J. N. | Zendy; Njagi E. N. M. | Zendy

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Anti‐HIV‐1 activities in extracts from some medicinal plants as assessed in an in vitro biochemical HIV‐1 reverse transcriptase assay

Author(s) -

Kanyara J. N.,

Njagi E. N. M.

Publication year - 2005

Publication title -

phytotherapy research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.019

H-Index - 129

eISSN - 1099-1573

pISSN - 0951-418X

DOI - 10.1002/ptr.1536

Subject(s) - reverse transcriptase , microtiter plate , in vitro , biology , enzyme , ic50 , human immunodeficiency virus (hiv) , medicinal plants , biochemistry , virology , microbiology and biotechnology , polymerase chain reaction , botany , gene

An in vitro HIV‐1 reverse transcriptase (RT) assay was used for screening of anti‐HIV activity of extracts obtained from some Kenyan medicinal plants. The assay utilises [ 3 H]‐methyl thymidine triphosphate (dTTP) as the enzyme substrate and polyadenylic acid.oligodeoxythymidylic acid [poly(rA).p(dT) 12–18 ] as the template‐primer dimmer. This assay was optimised and standardised with respect to the various experimental parameters in a microtiter plate methodology. The assay was then applied to test for potential antiviral activities of several Kenyan medicinal plant extracts and the concentrations producing 50% inhibition (IC 50 ) of the HIV‐1 RT were determined. This assay is described in this report and results obtained with some of the extracts are presented. Copyright © 2005 John Wiley & Sons, Ltd.

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