Premium
Identification of the antibacterial component of an ethanolic extract of the Australian medicinal plant, Eremophila duttonii
Author(s) -
Shah Arvind,
Cross Reg F.,
Palombo Enzo A.
Publication year - 2004
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.1507
Subject(s) - antibacterial activity , thin layer chromatography , chromatography , chemistry , flavonoid , traditional medicine , fractionation , pharmacognosy , column chromatography , bacteria , amorphophallus , food science , biology , biological activity , organic chemistry , biochemistry , medicine , genetics , in vitro , antioxidant
Activity‐guided fractionation was used to determine the antibacterial component of an ethanolic extract of the leaves of an Australian native medicinal plant, Eremophila duttonii F. Muell. (Myoporaceae). The extract, previously shown to have activity against Gram positive bacteria, was shown to have activity against additional Gram positive bacteria, including Clostridium perfringens , C. sporogenes and Listeria monocytogenes . Thin layer chromatography (TLC) was used to separate the extract into seven coloured fractions in visible light, one of which was shown by bioautography to contain antibacterial activity. Recovery of the component from the TLC plate and testing for antibacterial activity using a plate‐hole diffusion assay supported this result. The purity of the component was veried by high‐performance liquid chromatography and a time‐kill experiment indicated that the puried component showed identical bactericidal activity to the whole extract. TLC spray reagents indicated that the component was a sterol, terpene or sugar but not a avonoid, while the pigmented nature suggested a carotenoid. Copyright © 2004 John Wiley & Sons, Ltd.