Premium
Attenuating effect of a traditional korean formulation, Paeng‐Jo‐Yeon‐Nyeon‐Baek‐Ja‐In‐Hwan (PJBH), on hydrogen peroxide‐induced injury in PC12 cells
Author(s) -
Koo ByungSoo,
Kim YeonKye,
Park KyungSu,
Chung KangHyun,
Kim CheorlHo
Publication year - 2004
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.1457
Subject(s) - catalase , traditional medicine , lipid peroxidation , pharmacology , chemistry , antioxidant , ginseng , glutathione peroxidase , hydrogen peroxide , malondialdehyde , radix (gastropod) , neuroprotection , biochemistry , medicine , biology , botany , pathology , alternative medicine
Abstract The Paeng‐Jo‐Yeon‐Nyeon‐Baek‐Ja‐In‐Hwan (PJBH) prescription is a dried decoctum consisting of a mixture of 18 medicinal herbs that include Semen Biotae, Fructus Torilis seu cnidii, Fructus Rubi, Herba Dendrobii, Radix Morindae ofcinalis, Cortex Eucommiae, Radix Aspragi, Radix Polygalae, Radix Dipsaci, Ramulus Cinnamomi, Rhizoma Acori graminei, Rhizoma Alismatis, Rhizoma Dioscoreae, Radix Ginseng, Radix Rehmanniae preparata, Fructus corni, Fructus Schisandrae and Herba Cistanches. The effect of PJBH extracts on H 2 O 2 ‐induced toxicity in the rat pheochromocytoma line PC12 was examined by measurements of cell lesion, level of lipid peroxidation and antioxidant enzyme activities, since free radicals are involved in neurodegeneration in Alzheimer's disease (AD). After a 30 min exposure of the cells to H 2 O 2 (150 µM), a marked decrease in cell survival, activities of glutathione peroxidase and catalase as well as an increased production of malondialdehyde (MDA) were found. Pretreatment of the cells with PJBH (0.5–10 µg/ml) prior to H 2 O 2 exposure signicantly elevated cell survival, antioxidant enzyme activities and resulted in a decrease in the level of MDA. The effects of the PJBH on hydrogen peroxide‐induced injury in PC12 cells were also examined. PJBH had a remarkable elevating effect on catalase and GSH‐Px activities as well as cell survival, suggesting that cytoprotective effects of the PJBH are involved in stimulation against intermediate concentrations of H 2 O 2 ‐induced PC12 cell injury. The above‐mentioned neuroprotective effects were also compared with the effect of tacrine. The results suggest that PJBH has potential for use as a novel neuronal therapeutic agent. Copyright © 2004 John Wiley & Sons, Ltd.