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Inhibitory effects of leaf extracts of Stachytarpheta jamaicensis (Verbenaceae) on the respiratory burst of rat macrophages
Author(s) -
Álvarez Ezequiel,
Leiro José Manuel,
Rodríguez Melita,
Orallo Francisco
Publication year - 2004
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.1442
Subject(s) - verbenaceae , respiratory burst , lipopolysaccharide , nitric oxide , reactive oxygen species , pharmacology , xanthine oxidase , pharmacognosy , biochemistry , chemistry , biology , biological activity , traditional medicine , in vitro , medicine , immunology , endocrinology , enzyme
The anti‐oxidant effects of ethyl acetate (EAcE) and n ‐hexane extracts (nHE) of dried leaves of Stachytarpheta jamaicensis Vahl. (Verbenaceae) on the reactive oxygen species (ROS) generating during the respiratory burst of rat peritoneal macrophages were investigated. Only EAcE, at concentrations between 0.4 and 40 µg/ml, inhibited the extracellular release of oxygen radicals by resident peritoneal macrophages stimulated with phorbol‐12‐myristate 13‐acetate (PMA). At concentrations above 40 µg/ml, EAcE inhibited the production of nitric oxide (NO) in macrophages stimulated in vivo with sodium thioglycollate then in vitro with lipopolysaccharide (LPS) and gamma‐interferon (IFN‐ γ ). nHE extracts at concentrations between 0.4 and 40 µg/ml did not scavenge O − 2 generated enzymatically by hypoxanthine/xanthine oxidase (HX/XO) system, but EAcE at the same concentrations showed potent O − 2 ‐scavenging activity. At 40 µg/ml, EAcE also inhibited XO activity. These results suggest that the EAcE extract of S. jamaicensis may be a potential pharmaceutical value in treatment of immunopathological diseases related to oxidative stress. Copyright © 2004 John Wiley & Sons, Ltd.