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Stereospecific effects of catechin isomers on insulin induced lipogenesis in 3T3‐L1 cells
Author(s) -
Mochizuki Miyako,
Hasegawa Noboru
Publication year - 2004
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.1437
Subject(s) - lipogenesis , triglyceride , catechin , chemistry , endocrinology , medicine , lipid metabolism , insulin , intracellular , adipose tissue , metabolism , dehydrogenase , biochemistry , adipocyte , biology , enzyme , polyphenol , antioxidant , cholesterol
Abstract We studied the inuence of (+)‐ and (−)‐catechin contained in green tea on insulin induced lipogenesis in 3T3‐L1 cells. In 14 days of culture with insulin, the intracellular triglyceride concentration and the activities of glycerophosphate dehydrogenase, a marker of adipose conversion, increased. The addition of 0.02 mg/ml (+)‐isomer stimulated the accumulation of triglyceride induced by insulin, but the addition of the same concentration of (−)‐isomer inhibited lipogenesis. The activities of glycerophosphate dehydrogenase were changed by (+)‐ and (−)‐catechin in the same direction as the corresponding changes in triglyceride accumulation. These data suggest a biological signicance of catechins, with opposite effects on lipid metabolism depending on the isomer. Copyright © 2004 John Wiley & Sons, Ltd.