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Biological activity of barbados cherry (acerola fruits, fruit of Malpighia emarginata DC) extracts and fractions
Author(s) -
Motohashi Noboru,
Wakabayashi Hidetsugu,
Kurihara Teruo,
Fukushima Hidetaka,
Yamada Tomoko,
Kawase Masami,
Sohara Yoshitaka,
Tani Satoru,
Shirataki Yoshiaki,
Sakagami Hiroshi,
Satoh Kazue,
Nakashima Hideki,
Molnár Annamária,
Spengler Gabriella,
Gyémánt Nóra,
Ugocsai Katalin,
Molnár Joseph
Publication year - 2004
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.1426
Subject(s) - malpighiaceae , staphylococcus epidermidis , cytotoxic t cell , chemistry , ethyl acetate , biological activity , biology , microbiology and biotechnology , traditional medicine , biochemistry , bacteria , staphylococcus aureus , botany , in vitro , medicine , genetics
Fractionation of barbados cherry (acerola fruit, a fruit of Malpighia emarginata DC.) extracts were performed by organic solvent extractions and column chromatographies, using two extraction methods. Higher cytotoxic activity was concentrated in fractions A4 and A6 (acetone extract), and H3 and HE3 (hexane extract). These four fractions showed higher cytotoxic activity against tumor cell lines such as human oral squamous cell carcinoma (HSC‐2) and human submandibular gland carcinoma (HSG), when compared with that against normal cells such as human periodontal ligament broblasts (HPLF) and human gingival broblasts (HGF). HE2 (hexane extract), AE2 (ethyl acetate extract), AE3, AE4, AE5, A8, A9 and A10 showed some relatively higher anti‐bacterial activity on the Gram‐positive Staphylococcus epidermidis ATCC 1228 but were ineffective on the representative Gram‐negative species E. coli and Ps. aeruginosa . The fractions were inactive against Helicobacter pylori , two representative Candida species, and human immunodeciency virus (HIV). H3, H4 and HE3, which displayed higher tumor‐specic cytotoxicity also showed higher multidrug resistance (MDR) reversal activity, than (±)‐verapamil as positive control. ESR spectroscopy shows that the radical‐mediated oxidation is not involved in the induction of tumor‐specic cytotoxic activity. The tumor specic cytotoxic activity and MDR reversal activity of barbados cherry may suggest its possible application for cancer therapy. Copyright © 2004 John Wiley & Sons, Ltd.