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Antimycobacterial constituents from Juniperus procera, Ferula communis and Plumbago zeylanica and their in vitro synergistic activity with isonicotinic acid hydrazide
Author(s) -
Mossa Jaber S.,
ElFeraly Farouk S.,
Muhammad Ilias
Publication year - 2004
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.1420
Subject(s) - antimycobacterial , chemistry , traditional medicine , plumbagin , minimum inhibitory concentration , mycobacterium smegmatis , in vitro , microbiology and biotechnology , biochemistry , biology , botany , mycobacterium tuberculosis , tuberculosis , medicine , pathology
The synergistic activity of antimycobacterial constituents from Saudi plants was evaluated in combination with isonicotinic acid hydrazide (INH) against four atypical organisms, namely, Mycobacterium intracellulare, M. smegmatis, M. xenopei and M. chelonei . The potency of INH was increased four‐fold, using an in vitro checkerboard method, against each mycobacteria when tested with a subtoxic concentration of the totarol, isolated from J. procera . The MIC values of totarol, ferulenol (from Ferula communis ) and plumbagin (from Plumbago zeylanica ) were thus lowered from 1.25–2.5 to 0.15–0.3 µg/mL due to synergism with INH. When tested against the resistant strain of M. tuberculosis H37Rv, plumbagin and 7 β ‐hydroxyabieta‐8,13‐dien‐11,12‐dione exhibited inhibitory activity at <12.5 µg/mL, while others were inactive at this concentration. Copyright © 2004 John Wiley & Sons, Ltd.