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Chemoprotective effect of plant phenolics against anthracycline‐induced toxicity on rat cardiomyocytes. Part I. Silymarin and its flavonolignans
Author(s) -
Chlopčíková Šárka,
Psotová Jitka,
Miketová Petra,
Šimánek Vilím
Publication year - 2004
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.1415
Subject(s) - silybum marianum , silibinin , pharmacology , milk thistle , chemistry , oxidative stress , cytotoxicity , cytoprotection , doxorubicin , traditional medicine , viability assay , chemoprotective , antioxidant , biochemistry , medicine , cell , in vitro , surgery , chemotherapy
Abstract Silymarin, an extract of avonolignans from the dried fruits of milk thistle ( Silybum marianum L. Gaertneri) and its constituents silibinin, dehydrosilibinin, silychristin and silydianin were tested for protective effects on rat cardiomyocytes exposed to doxorubicin. Silymarin and individual avonolignans did not exert cytotoxicity in the range 25–100 µ m (incubation 9 h). Dehydrosilibinin was tested only at 25 µ m concentration due to its low solubility. All substances increased the cell ATP level. Silymarin and avonolignans displayed a dose‐dependent cytoprotection against doxorubicin (100 µ m , incubation 8 h). The protective effects of silymarin, silibinin, dehydrosilibinin and silychristin were comparable to that of dexrasoxane, while silydianin exerted the best protective effect. The ability of silymarin complex and its components to protect cardiomyocytes against doxorubicin‐induced oxidative stress is due mainly to their cell membrane stabilization effect, radical scavenging and iron chelating potency. Copyright © 2004 John Wiley & Sons, Ltd.