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Estimation of naphthaquinones from Arnebia hispidissima (Lehm.) DC. in vivo and in vitro . I. anti‐inflammatory screening
Author(s) -
Singh B.,
Sahu P. M.,
Jain S. C.,
Singh S.
Publication year - 2004
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.1385
Subject(s) - in vivo , boraginaceae , in vitro , arthritis , carrageenan , traditional medicine , potency , pharmacology , medicine , chemistry , biology , immunology , biochemistry , botany , microbiology and biotechnology
The hexane extract of Arnebia hispidissima yielded a mixture of naphthaquinones: arnebin‐1, arnebin‐7, tiglic acid (ester of dihydroxy alkannin), alkannin, arnebinol and cycloarnebin‐7. To evaluate the anti‐inammatory activities of hexane extract and isolated naphthaquinones, models with carrageenan‐induced acute arthritis and complete Freund's adjuvant (CFA)‐induced chronic arthritis in rats were conducted. The observed results indicated that pretreatment with cycloarnebin‐7 signicantly inhibited the carrageenan‐induced acute arthritis. Moreover, arnebin‐1, signicantly suppressed the development of chronic arthritis induced by CFA. The present study deals with the quantication of naphthaquinones from in vivo and in vitro cell cultures of plant species and isolated compounds from intact plant tested for their swelling inhibitory potency. It has been reported that arnebin‐1 was the major naphthaquinone both in vivo (0.62%) and in vitro (0.27%) cell cultures. Copyright © 2004 John Wiley & Sons, Ltd.