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Anti‐HIV‐1 limonoid: first isolation from Clausena excavata
Author(s) -
Sunthitikawinsakul Arunrat,
Kongkathip Ngampong,
Kongkathip Boonsong,
Phonnakhu Sida,
Daly John W.,
Spande Thomas F.,
Nimit Yuth,
Napaswat Chanita,
Kasisit Jittra,
Yoosook Chalobon
Publication year - 2003
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.1381
Subject(s) - limonoid , rhizome , chemistry , stereochemistry , traditional medicine , chloroform , rutaceae , ethanol , pharmacognosy , biological activity , biology , biochemistry , botany , in vitro , chromatography , medicine
Abstract A limonoid, clausenolide‐1‐ethyl ether (1) and two coumarins, dentatin (2) and nor ‐dentatin (3), were isolated from Clausena excavata . Limonoid 1 was obtained from the crude ethanol extract of the rhizomes and the roots but had not previously been isolated from C. excavata and exhibited HIV‐1 inhibitory activity. Coumarins 2 and 3, with their structures related to an anti‐HIV‐1 substance, (+)‐calanolide A (4), were obtained from the crude chloroform extract of the rhizomes. Both induced toxicity to cells used in a syncytium assay for anti‐HIV‐1 activity. These compounds, 1–3, did not show any cytotoxic effect against KB and BC‐1 cell lines (IC 50 value > 20 µg/mL). Copyright © 2003 John Wiley & Sons, Ltd.