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Anti‐inflammatory activity, cytotoxicity and active compounds of Tinospora smilacina Benth.
Author(s) -
Li Rachel W.,
Leach David N.,
Myers Stephen P.,
Leach Gregory J.,
Lin G. David,
Brushett Donald J.,
Waterman Peter G.
Publication year - 2004
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.1373
Subject(s) - cyclooxygenase , arachidonate 5 lipoxygenase , cytotoxicity , anti inflammatory , phospholipase a2 , pharmacology , menispermaceae , traditional medicine , in vitro , cytotoxic t cell , chemistry , biological activity , medicine , biochemistry , arachidonic acid , enzyme , stereochemistry , alkaloid
Tinospora smilacina Benth. has been used in Australian indigenous medicine for the treatment of headache, rheumatoid arthritis and other inammatory disorders. As part of an investigation into the anti‐inammatory potential of plants using an ethnopharmacological approach, the present study sought to evaluate the efcacy and safety of Tinospora smilacina . An ethanol extract of this plant was evaluated in vitro for anti‐inammatory activities on cyclooxygenase‐1 (COX‐1), cyclooxygenase‐2 (COX‐2), 5‐lipoxygenase (5‐LO) and phospholipase A 2 (PA 2 ). The ethanol extract of Tinospora smilacina showed inhibitory activities on COX‐1, COX‐2, 5‐LO and PA 2 with the IC 50 values of 63.5, 81.2, 92.1 and 30.5 µg/mL respectively. Cytotoxic effect of the extracts of Tinospora smilacina was investigated in vitro using ATP‐based luminescence assay and the results showed no cytotoxic effect on cell lines of skin broblasts (1BR3), human Caucasian hepatocyte carcinoma (Hep G2) and human Caucasian promyelocytic leukaemia (HL‐60). This paper also describes the results of fractionations and bioassay guided chemical studies, suggesting that the anti‐inammatory activity is due to triterpene‐fatty acid esters and free fatty acids. Copyright © 2004 John Wiley & Sons, Ltd.