Premium
The effect of medicinal plants of Islamabad and Murree region of Pakistan on insulin secretion from INS‐1 cells
Author(s) -
Hussain Zakir,
Waheed Abdul,
Qureshi Rizwana Aleem,
Burdi Dadu Khan,
Verspohl Eugen J.,
Khan Naeema,
Hasan Mashooda
Publication year - 2004
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.1372
Subject(s) - traditional medicine , cassia , polygonum , medicinal plants , biology , botany , secretagogue , moringa , chemistry , insulin , medicine , microbiology and biotechnology , alternative medicine , pathology , traditional chinese medicine
In vitro testing of the extracts of medicinal plants collected from Islamabad and the Murree region on insulin secretagogue activity was carried out. Dried ethanol extracts of all plants (ZH1‐ZH19) were dissolved in ethanol and DMSO, and tested at various concentrations (between 1 and 40 µg/mL) for insulin release from INS‐1 cells in the presence of 5.5 mM glucose. Glibenclamide was used as a control. Promising insulin secretagogue activity in various plant extracts at 1, 10, 20 and 40 µg/mL was found, while in some cases a decrease in insulin secretion was also observed. Artemisia roxburghiana , Salvia coccinia and Monstera deliciosa showed insulin secretagogue activity at 1 µg/mL ( p < 0.05) while Abies pindrow , Centaurea iberica and Euphorbia helioscopia were active at 10 µg/mL ( p < 0.05). Extracts of Bauhinia variegata and Bergenia himalacia showed effects at 20 µg/mL ( p < 0.05), and Taraxacum ofcinale and Viburnum foetens at 40 µg/mL ( p < 0.05). Insulin secretagogue activity could not be detected in the extracts of Adhatoda vasica , Cassia stula , Chrysanthemum leucanthemum , Morus alba , Plectranthus rugosus , Peganum harmala and Olea ferruginea . The results suggest that medicinal plants of Islamabad and the Murree region of Pakistan may be potential natural resources for antidiabetic compounds. Copyright © 2004 John Wiley & Sons, Ltd.