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Emblica officinalis causes myocardial adaptation and protects against oxidative stress in ischemic‐reperfusion injury in rats
Author(s) -
Rajak S,
Banerjee S. K.,
Sood S,
Dinda A. K.,
Gupta Y. K.,
Gupta S. K.,
Maulik S. K.
Publication year - 2004
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.1367
Subject(s) - tbars , oxidative stress , glutathione peroxidase , catalase , antioxidant , superoxide dismutase , thiobarbituric acid , lipid peroxidation , reperfusion injury , pharmacology , glutathione , medicine , reactive oxygen species , chemistry , ischemia , biochemistry , enzyme
The fruits of Emblica ofcinalis (Amla) are widely used in the Indian System of Medicine and are believed to increase defense against disease. In the present study, the effects of chronic oral administration of fresh fruit homogenate of Amla on: (i) myocardial antioxidant system and (ii) oxidative stress induced by ischemic‐reperfusion injury (IRI) in rat heart were investigated. Fresh amla fruit homogenate, in three different doses (250, 500 and 750 mg/kg) and normal saline (C) were administered orally to Wistar albino rats (120–150 gms) of either sex daily for 30 days. There was reduction in basal myocardial lipid peroxidation, as evidenced by decreased thiobarbituric acid reactive substances (TBARS) level, and augmentation of myocardial endogenous antioxidants, like superoxide dismutase (SOD), catalase and glutathione peroxidase (GPx) in the treated rats. Hearts were also subjected to in vitro IRI (9 min of global ischemia, followed by 12 min of reperfusion, Langendorff's mode). Signicant myocyte injury and rise in myocardial TBARS along with depletion of SOD, catalase, GSH (reduced glutathione) and GPx occurred in the control group. No signicant increase in myocardial TBARS and depletion of antioxidant enzymes were observed in the treated groups. Myocyte injury was evident only in 250 mg/kg group. The results indicate that chronic Emblica ofcinalis administration causes myocardial adaptation by augmenting endogenous antioxidants and protects rat hearts from oxidative stress associated with ischemic‐reperfusion injury. Copyright © 2004 John Wiley & Sons, Ltd.