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Reporter gene assays and their applications to bioassays of natural products
Author(s) -
New D. C.,
MillerMartini D. M.,
Wong Y. H.
Publication year - 2003
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.1312
Subject(s) - bioassay , reporter gene , drug discovery , computational biology , biology , high throughput screening , bioreporter , microbiology and biotechnology , natural product , broad spectrum , gene , biochemical engineering , chemistry , biochemistry , combinatorial chemistry , gene expression , genetics , engineering
Medicinal plants represent precious resources from which bioactive compounds can be isolated and developed into invaluable therapeutic agents. With the advent of modern drug discovery technologies such as combinatorial chemistry and high‐throughput drug screening platforms, there is an increasing interest in utilizing medicinal plants as a source of drug leads. A wide spectrum of bioassays can be employed for the detection of bioactivity in extracts, fractions, as well as puried compounds of herbal origin. Amongst the different types of bioassays, reporter gene assays are highly versatile and reliable. The present review provides an overview of the most popular reporter genes in terms of their basic methodology, capacities and limitations. The different types of intracellular and extracellular reporter gene products and their potential applications in bioassays of natural products are also discussed. Copyright © 2003 John Wiley & Sons, Ltd.