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In vitro antiviral activity of Phyllanthus orbicularis extracts against herpes simplex virus type 1
Author(s) -
Fernández Romero J. A.,
del Barrio Alonso G.,
Romeu Álvarez B.,
Gutiérrez Y.,
Valdés V. S.,
Parra F.
Publication year - 2003
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.1300
Subject(s) - herpes simplex virus , vero cell , virus , in vitro , biology , virology , cell culture , extracellular , chemistry , microbiology and biotechnology , traditional medicine , stereochemistry , biochemistry , medicine , genetics
The antiviral activity of butanol‐ and acetic acid‐soluble fractions, prepared from the leaves and stems of Phyllanthus orbicularis H.B.K., has been investigated against acyclovir‐sensitive or ‐resistant herpes simplex virus type 1 (HSV‐1) strains, using human foreskin broblast (HFF) and green ape kidney (Vero) cell lines. Both fractions showed antiviral selectivity indexes (SI) from 10.3 to 22.8, while their extracellular virucidal activities reached SI values ranging from 371 to 1040. Time‐addition experiments suggested that the active compounds present in the studied fractions acted on early steps of the virus replication cycle. Copyright © 2003 John Wiley & Sons, Ltd.