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Plasma lipoproteins in transport of silibinin, an antioxidant flavonolignan from Silybum marianum
Author(s) -
Švagera Zdeněk,
Škottová Nina,
Váňa Petr,
Večeřa Rostislav,
Urbánek Karel,
Belejová Marie,
Kosina Pavel,
Šimánek Vilím
Publication year - 2003
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.1187
Subject(s) - silibinin , silybum marianum , chemistry , lipoprotein , antioxidant , phosphatidylcholine , albumin , chylomicron , very low density lipoprotein , biochemistry , cholesterol , pharmacology , biology , phospholipid , medicine , traditional medicine , membrane
To assess the role of plasma lipoproteins in the transport of silibinin, an antioxidant avonolignan, 125 I‐labelled silibinin ( 125 I‐SB) administered perorally to the rat was used. The plasma 125 I‐SB derived radioactivity was distributed among plasma lipoproteins according to their lipophilicity (TAG‐rich lipoproteins 30–40% > LDL 15% > HDL 5%), and in the fraction of d > 1.215 containing albumin and other proteins a minority amount of radioactivity was found. Administration of 125 I‐SB in a complex with phosphatidylcholine resulted in proportionally higher radioactivities in all fractions as well as in tissues. Dietary olive oil had a slightly decreasing effect on plasma concentrations of silibinin measured by HPLC as well as on 125 I‐SB derived radioactivity in plasma and liver. In the TAG‐rich lipoprotein fraction and HDL no effects of olive oil on the levels of 125 I‐SB derived radioactivities were observed, however, at a 30 min interval the levels of 125 I‐SB derived radioactivity in LDL and the heart were signicantly decreased in the olive oil group. These results suggest that (i) silibinin is not resorbed by the chylomicron pathway, and (ii) the endogenous lipoprotein pathway VLDL → LDL may play a role in the transport of silibinin from the liver to the extrahepatic tissues concurrently facilitating the lipoprotein antioxidant inuence of silibinin. Copyright © 2003 John Wiley & Sons, Ltd.