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Protection of brain cells against AMPA‐induced damage by Asiasari radix extracts
Author(s) -
Han Yong,
Kwon Eun Hee,
Kim SungJin
Publication year - 2003
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.1176
Subject(s) - depolarization , ampa receptor , chemistry , fraction (chemistry) , inhibitory postsynaptic potential , methanol , biochemistry , biophysics , pharmacology , biology , chromatography , glutamate receptor , neuroscience , organic chemistry , receptor
We determined whether Asiasari Radix (AR) extracts have protective actions in brain cells. Methanol extracts of Asiasari Radix (fraction 1) have signicant inhibitory effects on the AMPA‐induced rat cortical depolarization in the grease gap assay. In differentiated PC12 cells, it almost completely protected against AMPA‐induced cell death. In addition, it had some protective actions in C6 glial cells death induced by AMPA. The methanol extracts (fraction 1) of AR were subsequently fractionated into chloroform‐(fraction 2), chloroform/methanol‐(3:1) (fraction 3), methanol‐soluble (fraction 4) and methanol‐insoluble, water‐soluble fractions (fraction 5). Among these, fraction 4 had the strongest inhibitory effects against AMPA‐induced cell death in the PC 12 cells and also dramatically inhibited AMPA‐induced depolarization of rat brain cortex in the grease gap assay. Interestingly, fraction 4 blocked the Zn‐induced oxidative damages in C6 glial cells. Copyright © 2003 John Wiley & Sons, Ltd.