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Two isoflavones and bioactivity spectrum of the crude extracts of Iris germanica rhizomes
Author(s) -
Orhan Ilkay,
Nasim Shama,
Tener Bilge,
Ayanoglu Filiz,
Özgüven Mensure,
Choudhary M. Iqbal
Publication year - 2003
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.1169
Subject(s) - phytotoxicity , brine shrimp , petroleum ether , ethyl acetate , chemistry , chloroform , toxicity , rhizome , traditional medicine , botany , biology , chromatography , organic chemistry , medicine , extraction (chemistry)
Abstract In vitro biological activities including bactericidal, fungicidal and insecticidal activities as well as phytotoxicity and brine shrimp toxicity of the petroleum ether, chloroform and ethyl acetate extracts of Iris germanica L. were determined. The bactericidal activity of the extracts was assayed by the agar well diffusion test. In the fungicidal test, the agar tube dilution method was used. The insecticidal activity was determined by the exposure method. The toxicity of the extracts was evaluated by the phytotoxicity test as well as the brine shrimp toxicity test. The chloroform and ethyl acetate extracts of I. germanica rhizomes exhibited bactericidal activity, while the petroleum ether extract did not exhibit any bactericidal, fungicidal and insecticidal activities. It was also inactive in the brine shrimp toxicity test, whereas it showed signicant phytotoxicity against the plant Lemna aequinoctialis Welv. Two known isoavones were isolated from the chloroform extract of the plant. Copyright © 2003 John Wiley & Sons, Ltd.