Premium
Butanolides from Machilus thunbergii and their inhibitory activity on nitric oxide synthesis in activated macrophages
Author(s) -
Kim Nam Yi,
Ryu JaeHa
Publication year - 2003
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.1160
Subject(s) - nitric oxide , inhibitory postsynaptic potential , nitric oxide synthase , chemistry , pharmacognosy , traditional medicine , biology , biochemistry , in vitro , biological activity , medicine , organic chemistry , endocrinology
In activated macrophages the inducible form of nitric oxide synthase (iNOS) generates high amounts of the toxic mediator, nitric oxide (NO), that contributes to the circulatory failure associated with septic shock. Three butanolides were isolated from Machilus thunbergii as active principles which inhibit the production of NO in lipopolysaccharide‐activated RAW 264.7 cells, and their structures were identied as litsenolide A2 (1), B1 (2) and B2 (3). They showed dose‐dependent inhibition of NO syntheses and the IC 50 s were 3.36, 3.70 and 6.19 µ m , respectively. These new inhibitors of iNOS may have potential in the treatment of endotoxaemia and inammation accompanied by the overproduction of NO. Copyright © 2003 John Wiley & Sons, Ltd.