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Glutathione S‐transferases and malondialdehyde in the liver of NOD mice on short‐term treatment with plant mixture extract P‐9801091
Author(s) -
Petlevski R.,
Hadžija M.,
Slijepčević M.,
Juretić D.,
Petrik J.
Publication year - 2003
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.1128
Subject(s) - achillea millefolium , malondialdehyde , lipid peroxidation , glutathione , oxidative stress , biochemistry , chemistry , traditional medicine , cichorium , antioxidant , pharmacology , biology , botany , medicine , enzyme
Changes in the concentration of glutathione S‐transferases (GSTs) and malondialdehyde (MDA) were assessed in the liver of normal and diabetic NOD mice with and without treatment with the plant extract P‐9801091. The plant extract P‐9801091 is an antihyperglycaemic preparation containing Myrtilli folium (Vaccinium myrtillus L.), Taraxaci radix (Taraxacum ofcinale Web.), Cichorii radix (Cichorium intybus L.), Juniperi fructus (Juniperus communis L.) , Centaurii herba (Centaurium umbellatum Gilib.), Phaseoli pericarpium (Phaseolus vulgaris L.), Millefolii herba (Achillea millefolium L.), Mori folium (Morus nigra L.), Valerianae radix (Valeriana ofcinalis L.) and Urticae herba et radix (Urtica dioica L). Hyperglycaemia in diabetes mellitus is responsible for the development of oxidative stress (via glucose auto‐oxidation and protein glycation), which is characterized by increased lipid peroxide production (MDA is a lipid peroxidation end product) and/or decreased antioxidative defence (GST in the liver is predominantly an α enzyme, which has antioxidative activity). The catalytic concentration of GSTs in the liver was signicantly reduced in diabetic NOD mice compared with normal NOD mice ( p < 0.01), while the concentration of MDA showed a rising tendency (not signicant). The results showed that statistically signicant changes in antioxidative defence occurred in the experimental model of short‐term diabetes mellitus. A 7‐day treatment with P‐9801091 plant extract at a dose of 20 mg/kg body mass led to a signicant increase in the catalytic concentration of GSTs in the liver of diabetic NOD mice ( p < 0.01) and a decrease in MDA concentration (not signicant), which could be explained by its antihyperglycaemic effect. Copyright © 2003 John Wiley & Sons, Ltd.