Premium
Protective effect of a polyherbal formulation (Immu‐21) against cyclophosphamide‐induced mutagenicity in mice
Author(s) -
Jena G. B.,
Nemmani Kumar V. S.,
Kaul C. L.,
Ramarao P.
Publication year - 2003
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.1125
Subject(s) - withania somnifera , genotoxicity , cyclophosphamide , micronucleus test , ocimum , pharmacology , bone marrow , chemistry , traditional medicine , micronucleus , biology , medicine , toxicity , immunology , chemotherapy , alternative medicine , organic chemistry , pathology
The object was to evaluate the effects of a polyherbal formulation, Immu‐21, against cyclophosphamide (CP)‐induced chromosomal aberrations (CA) and micronuclei (MN) in mice. CP alone (40 mg/kg, i.p.) produced classical as well as non‐classical chromosomal aberrations in mice, and the incidence of CA was signicantly more in the CP treated group when compared with that of the control group. Immu‐21, which contains extracts of Ocimum sanctum , Withania somnifera , Emblica officinalis and Tinospora cordifolia , was given at 100 mg/kg, daily, over 7 days, and 30 mg/kg daily over 14 days and inhibited both CP‐induced classical and non‐classical chromosomal aberrations (∼40%–60% of control). A significant increase in MN was also observed in bone marrow erythrocytes of mice treated with CP, and pretreatment with Immu‐21 also signicantly reduced these. Cytotoxicity was evaluated by estimating the ratio of polychromatic erythrocytes (PCEs) to normochromatic erythrocytes (NCEs). The present results indicate that chronic treatment with Immu‐21 prevented CP‐induced genotoxicity in mice. Copyright © 2003 John Wiley & Sons, Ltd.